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il 18r13  (R&D Systems)


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  • 90

    Structured Review

    R&D Systems il 18r13
    Il 18r13, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/il+18r13/Recombinant+Human+IL-18+R+beta%2FIL-1+R7+Fc+Chimera%2C+CF/us08883975-587-73-74
    Average 90 stars, based on 2 article reviews
    il 18r13 - by Bioz Stars, 2026-09
    90/100 stars

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    Recombinant:

    Article Title: Antibodies against IL-18R1 and uses thereof
    Article Snippet: .. Thereafter the wells were blocked with PBS supplemented with 0.1% Tween®-20 and 2% BSA (Roche Diagnostics GmbH, DE) for 1 h. 0.2 μg/ml of recombinant human IL-18Rα Fc chimera (R&D Systems, UK, Cat. No. 816-LR) was captured for 1 h. Dilutions of purified antibodies in PBS with 0.5% BSA and 0.05% Tween®-20 were incubated with the receptor protein for 1 h. Biotinylated human IL-18 (MBL International, US, Cat. No. B003-5) and 0.2 μg/ml IL-18R13 (R&D Systems, UK, Cat. No. 118-AP) were added for an additional hour to build up the trimeric complex. .. IL-18 was biotinylated with Sulfo-NHS-LC-Biotin (Thermo Scientific Pierce, US, Cat. No. 21327) according to the manufacturer's protocol and purified using ZebaTM Desalt Spin Column (Thermo Scientific Pierce, US, Cat. No. 89889).

    Purification:

    Article Title: Antibodies against IL-18R1 and uses thereof
    Article Snippet: .. Thereafter the wells were blocked with PBS supplemented with 0.1% Tween®-20 and 2% BSA (Roche Diagnostics GmbH, DE) for 1 h. 0.2 μg/ml of recombinant human IL-18Rα Fc chimera (R&D Systems, UK, Cat. No. 816-LR) was captured for 1 h. Dilutions of purified antibodies in PBS with 0.5% BSA and 0.05% Tween®-20 were incubated with the receptor protein for 1 h. Biotinylated human IL-18 (MBL International, US, Cat. No. B003-5) and 0.2 μg/ml IL-18R13 (R&D Systems, UK, Cat. No. 118-AP) were added for an additional hour to build up the trimeric complex. .. IL-18 was biotinylated with Sulfo-NHS-LC-Biotin (Thermo Scientific Pierce, US, Cat. No. 21327) according to the manufacturer's protocol and purified using ZebaTM Desalt Spin Column (Thermo Scientific Pierce, US, Cat. No. 89889).

    Incubation:

    Article Title: Antibodies against IL-18R1 and uses thereof
    Article Snippet: .. Thereafter the wells were blocked with PBS supplemented with 0.1% Tween®-20 and 2% BSA (Roche Diagnostics GmbH, DE) for 1 h. 0.2 μg/ml of recombinant human IL-18Rα Fc chimera (R&D Systems, UK, Cat. No. 816-LR) was captured for 1 h. Dilutions of purified antibodies in PBS with 0.5% BSA and 0.05% Tween®-20 were incubated with the receptor protein for 1 h. Biotinylated human IL-18 (MBL International, US, Cat. No. B003-5) and 0.2 μg/ml IL-18R13 (R&D Systems, UK, Cat. No. 118-AP) were added for an additional hour to build up the trimeric complex. .. IL-18 was biotinylated with Sulfo-NHS-LC-Biotin (Thermo Scientific Pierce, US, Cat. No. 21327) according to the manufacturer's protocol and purified using ZebaTM Desalt Spin Column (Thermo Scientific Pierce, US, Cat. No. 89889).



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    90
    R&D Systems il 18r13
    Il 18r13, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/il+18r13/Recombinant+Human+IL-18+R+beta%2FIL-1+R7+Fc+Chimera%2C+CF/us08883975-587-73-74
    Average 90 stars, based on 1 article reviews
    il 18r13 - by Bioz Stars, 2026-09
    90/100 stars
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